Features & Benefits

AmplideX technology has become widely established for the detection and quantification of repeat expansions in a number of genes, including FMR1, C9orf72, and DMPK.  With the AmplideX PCR/CE SMN1 Kit, this approach is applied to copy number resolution, allowing SMN1 exon 7 copy number to be estimated in under four hours using readily available laboratory equipment.

Reduced Complexity

• Similar workflow to the AmplideX FMR1 kit*^† eases implementation and testing
• Scalable design allows for high-throughput testing
• Automated analysis objectively reports copy number

Optimized Workflow

• A simplified workflow compared to alternative methods
• Fully kitted solutions with fewer QC and pipetting steps
• DNA to data in under four hours with only 45 minutes of hands-on-time

Quality Performance

• Ability to differentiate between 0, 1, 2, 3 and > 3 copies
• Robust, reliable results with non-overlapping ratios for each copy number output
• Concordance with previously characterized samples

*For Research Use Only. Not for use in Diagnostic procedures.^†CE-IVD for US Export Only.

Analytical Characteristics

Analytical characteristics of AmplideX PCR/CE SMN1 Kit:

• Excellent concordance with previously characterized clinical samples (Figure 1)
• Consistent results compared to other commercially available methods (Figure 2)
• Automated results reporting via AmplideX Reporter software (Figure 3)

Figure 1. Excellent concordance with previously characterized clinical samples.

Figure 2: Consistent results compared to other commercially available methods.

Figure 3: Automated results reporting via AmplideX Reporter software.

Figure 4: Workflow for the AmplideX PCR/CE SMN1 Kit

Analytical Characteristics